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National Repository of Grey Literature 1 records found  Search took 0.01 seconds. 
Studies of Protein-DNA Interactions using Immunoprecipitation with DNA Probes Labelled with Electroactive Groups
 Hermanová, Monika ; Špaček, Jan ; Pivoňková, Hana ; Fojta, Miroslav
Different electrochemically active species were tested for labelling of DNA probes and detection of DNA-protein binding. As the DNA probes, oligonucleotides bearing or lacking specific binding site of the p53 protein were chosen. They were tail-labelled either via chemical modification of single-stranded (ss) oligo(dT) DNA tails with an oxoosmium complex, or via enzymatic synthesis of the ss tail by terminal deoxynucleotidyl transferase and deoxynucleoside triphosphates modified with electrochemically reducible moieties. Electrochemical detection enabled to discriminate between sequence-specific and non-specific p53-DNA binding.
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